| 摘要 |
The present invention relates to a method of producing a transgenic plant expressing a protein variant having modified immunogenicity as compared to the parent protein comprising the steps obtaining antibody binding peptide sequences in-volved in antibody binding, using the sequences to localise epitope sequences on the primaery and/or the 3-dimensional structure of parent protein, defining an epitope area includ-ing amino acids situated within 5 ANGSTROM from the epitope amino ac-ids constituting the epitope sequence, changing one or more of the amino acids defining the epitope area of the parent pro-tein by genetical engineering mutations of a DNA sequence en-coding the parent protein, introducing the mutated DNA se-quence into a suitable host, culturing said host and express-ing the protein variant, evaluating the immunogenicity of the protein variant using the parent protein as reference, intro-ducing the mutated DNA sequence into an expression construct and transforming a suitable plant cell with the construct, and regenerating the plant from the plant cell. The invention will provide less allergenic foods. |
