3-Phosphoglycerate dehydrogenase variants whose inhibition by L-serine is reduced, and genes encoding them

摘要

New 3-phosphoglycerate dehydrogenase (PGD) variant corresponds to wild type Escherichia coli PGD up to position 349, which is occupied by glutamic acid. New 3-phosphoglycerate dehydrogenase (PGD) variant corresponds to wild type Escherichia coli PGD with SEQ ID NO:2 (defined sequence of 410 amino acids given in the specification) up to position 349, which is occupied by glutamic acid. Independent claims are also included for: (1) serA allele coding for (I); (2) vector comprising a serA allele as above under the control of a promoter; (3) producing a microbial strain by introducing a vector as above into a microbial strain; (4) microbial strain for fermentative production of amino acids of the phosphoglycerate family or compounds derived from C1 metabolism, comprising (I); (5) producing amino acids of the phosphoglycerate family or compounds derived from C1 metabolism by culturing a microbial strain as above.