| 摘要 |
An enantioselective hydrolysis method of asymmetric diester compounds is provided to improve purity and yield of a target compound and inhibit environmental pollution by using a biocatalytic enzyme, so that the method is useful for mass production of intermediates in synthesis of lercanidipine. A (4S)-1,4-dihydro-2,6-dimethyl-5-methoxycarbonyl-4-(3-nitrophenyl)pyridine-3-carboxylic acid isomer represented by the formula(1) is prepared by enantioselective hydrolysis of methyl pivaloyloxymethyl 1,4-dihydro-2,6-dimethyl-4-(3-nitrophenyl) pyridine-3,5-dicarboxylate represented by the formula(2) in a solvent selected from a mixture containing water and C2-C8 dialkylether by using lipase derived from Pseudomonas cepacia at 15-30 deg. C. Further, the solvent additionally comprises acetone, and the dialkylether organic solvent is dialkylether or diisopropylether. |
