| 摘要 |
<p>A dye couple, comprising 3-methyl-2-benzothiazolinone hydrazone (MBTH) and 8-anilino-1-naphthalenesulfonate (ANS), is used as an indicator in a reaction cascade pro- ducing a strong oxidizing agent, such as hydrogen peroxide or other peroxides or perborates. The strong oxidizing agent reacts with the dye couple to produce a blue dye stuff. The MBTH-ANS dye couple exhibits strong and flat spectral absorption at the region of about 600 to 650 nm. This region is free of blood color interference, which enables one to measure glucose and other analytes that react with an oxidase enzyme to produce the strong oxidiz- ing agent, through the use of LED optics, accurately with- out much optic calibration. Further, the MBTH and ANS are very soluble in aqueous solution, yet become insoluble upon oxidative coupling. The poor solubility minimizes dye fad- ing, thus providing a stable endpoint.</p> |
