Sulfonamide Derivatives of Xanthene Compounds

摘要

Compounds having the general of formula I and/or formula II wherein R¹ and R² are the same or different and are independently selected from the group consisting of -H, -C₁-C₁₈-alkyl or -omega-sulfoalkyl; X and Y are the same or different and are independently selected from the group consisting of -O^-, -OH, -SH, -NH-NH₂, -F, -Cl, -Br, -I, -O-Su (succinimidyl/sulfosuccinimidyl), -O-STP (4-sulfo-2,3,5,6-tetrafluorophenyl), -O-TFP (2,3,5,6-tetrafluorophenyl), -O-benzotriazole, -benzotriazole, -NR-CO-CH₂-I, -NR₂, -NR-biomolecule, -NR-L-COO^-, -NR-L-COOH, -NR-L-COO-Su, -NR-L-COO-STP, -NR-L-COO-TFP, -NR-L-CONR₂, -NR-L-CO-biomolecule, -NR-L-CO-NH-NH₂, -NR-L-OH, -NR-L-O-phosphoramidite, -NR-L-CHO, -NR-L-maleimid, or -NR-L-NH-CO-CH₂-I; where R is equal to R¹ and R² and L is selected from the group consisting of a divalent linear (-(CH₂)_o-, o=1 to 15), crossed, or cyclic alkane group that can be substituted by at least one atom selected from the group consisting of oxygen, substituted nitrogen, and/or sulfur, and the biomolecule is a protein, antibody, nucleotide, oligonucleotide, biotin, or hapten; Z is -O^- or OH; U is -O^-, -OH, or NH-L-SO₂Z; Kat is Li, Na, K, ammonium (mono-, di- or trialkyl) or another cation; An is F, Cl, Br, I, BF₄, ClO₄, CH₃CO₂, CF₃CO₂ or another anion; m is an integer from 1-6 necessary to compensate the negative or positive charge from the dye moiety in formula I or formula II; and n is an integer from 0-12; compositions containing these compounds, and methods using these compounds, are disclosed.