发明名称 |
METHOD FOR DETERMINATION OF HIGH-DENSITY LIPOPROTEIN CHOLESTEROL, AND REAGENT FOR THE METHOD |
摘要 |
<p>[PROBLEMS] To provide: a method for the determination of a high-density lipoprotein (HDL) cholesterol level in a biological sample such as a serum and a plasma; and a reagent for use in the method. [MEANS FOR SOLVING PROBLEMS] Disclosed is a method for the determination of a cholesterol level in a high-density lipoprotein by an enzymatic reaction, which comprises: a first reaction step for preferentially scavenging cholesterol in a low-density lipoprotein, a very-low-density lipoprotein and chylomicron contained in a sample, wherein the first reaction is carried out in a buffer solution containing a cholesterol esterase, a cholesterol oxidase and a substance capable of inhibiting the reactivities of the enzymes against the high-density lipoprotein; and a second reaction step for determining the cholesterol level in the high-density lipoprotein, wherein the second reaction is carried out in a buffer solution which is the same as the buffer solution used in the first reaction step except that a substance capable of reducing or eliminating the inhibitory activities of the enzymes against cholesterol in the high-density lipoprotein in the first reaction is additionally contained.</p> |
申请公布号 |
WO2008087895(A1) |
申请公布日期 |
2008.07.24 |
申请号 |
WO2008JP50236 |
申请日期 |
2008.01.11 |
申请人 |
TOYO BOSEKI KABUSHIKI KAISHA;KIMATA, SHINSUKE;YONEDA, KEIZO |
发明人 |
KIMATA, SHINSUKE;YONEDA, KEIZO |
分类号 |
C12Q1/60;C12Q1/26;C12Q1/44;G01N33/92 |
主分类号 |
C12Q1/60 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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