发明名称 |
BINDING SURFACES FOR AFFINITY ASSAYS |
摘要 |
Non-saturated or non-saturated and orientated binding surfaces are provided, and methods and compositions for making non-saturated or non-saturated and orientated binding surfaces. Methods of making binding surfaces for preparing a support surface for an affinity assay are provided, including using non-saturated or non-saturated and orientated binding surfaces on paramagnetic microparticles. The methods include methods for making ligand::support coupler-based complexes by a process optionally employing a lowjnput ratio of ligand to support coupler, by dilution, and by methods employing a dispersion and/or coating step using a block copolymer. Specific examples employing biotin-BSA and biotin-ovalbumin binding surfaces are provided, as well as strepavidin-coated microparticles and microparticles coated with capture moieties such as, for example, biotinylated immunoglobulins or fragments thereof. Other examples couple a ligand to the solid surface. Use of the methods and compositions in connection with a wide variety of analytes and capture moieties is provided. Non-saturated or non- saturated and orientated binding surfaces for immunoassays are provided. Further provided are dispersed microparticles and methods for making them.
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申请公布号 |
WO2008057366(A8) |
申请公布日期 |
2008.07.24 |
申请号 |
WO2007US23033 |
申请日期 |
2007.11.01 |
申请人 |
BECKMAN COULTER, INC.;SOLDO, JOSHUA, C.;SACKRISON, JAMES, L. |
发明人 |
SOLDO, JOSHUA, C.;SACKRISON, JAMES, L. |
分类号 |
G01N33/53;B01L99/00 |
主分类号 |
G01N33/53 |
代理机构 |
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