METHOD FOR DETERMINATION OF LOW-DENSITY LIPOPROTEIN CHOLESTEROL, AND REAGENT FOR THE METHOD

摘要

[PROBLEMS] To provide: a method for the determination of a low-density lipoprotein (LDL) cholesterol level in a biological sample such as a serum and a plasma; and a reagent for use in the method. [MEANS FOR SOLVING PROBLEMS] Disclosed is a method for the determination of a cholesterol level in a low-density lipoprotein by an enzymatic reaction, which comprises: a first reaction step for preferentially scavenging cholesterol in a high-density lipoprotein, a very-low-density lipoprotein and chylomicron contained in a sample, wherein the first reaction is carried out in a buffer solution containing a cholesterol esterase, a cholesterol oxidase, a substance capable of inhibiting the reactivities of the enzymes against the low-density lipoprotein, casein, and/or a quaternary ammonium salt, and/or a betaine compound; and a second reaction step for determining the cholesterol level in the low-density lipoprotein, wherein the second reaction is carried out in a buffer solution which is the same as the buffer solution used in the first reaction step except that a substance capable of reducing or eliminating the inhibitory activities of the enzymes against cholesterol in the low-density lipoprotein in the first reaction is additionally contained.