| 摘要 |
PROBLEM TO BE SOLVED: To provide both a method for preparing a self-assembly having a shortened reactional time, an extended optimum reactional temperature region and an improved operating efficiency and applicability and a simple method for detecting a gene utilizing the method. SOLUTION: This method for preparing the self-assembly comprises using a plurality of pairs of probes for forming a dimer in which each of a pair of oligonucleotides is divided into three regions of a 3'-terminal region, the central region and a 5'-terminal region and base sequences in the central region of the oligonucleotides are made mutually complementary while base sequences in the 3'-terminal region and the 5'-terminal region are made mutually noncomplementary and a plurality of pairs of cross- linking probes in which each of a pair of oligonucleotides is divided into two regions of a 3'-terminal region and a 5'-terminal region and base sequences in the 3'-terminal region and the 5'-terminal region are made mutually noncomplementary, converting the cross-linking probes into the base sequences capable of cross-linking the dimer formed from the probes for forming the dimer and thereby hybridizing the probes. As a result, oligonucleotides are self-assembled and a double-stranded regular high-order structure is formed. |
