| 摘要 |
<p><P>PROBLEM TO BE SOLVED: To provide a technology for preparing human ES cells (embryonic stem cells), which can clear an ethical problem and a technology related to the preparation technology. <P>SOLUTION: A somatic cell-derived nucleus is transplanted into an enucleated unfertilized egg to form a cloned embryo and the cloned embryo is treated to generate a divided embryo. Then, the blastomere cells of the divided embryo generated by, e.g. an 8 cell period are co-cultured together with separately prepared ES cells of the same kind of animal species so that divided embryo-derived ES cells, namely, blastomere cell-derived ES cells are induced and prepared. Then, the cloned ES cells are separately cultured so as to establish cloned ES cell strains. <P>COPYRIGHT: (C)2008,JPO&INPIT</p> |
