| 摘要 |
An isolated GRP94 ligand binding domain polypeptide. In some embodiments, the isolated GRP94 ligand binding domain polypeptide includes (a) an amino acid sequence encoded by a nucleic acid molecule that includes a nucleotide sequence selected from among: (i) nucleotides 310-1116 of SEQ ID NO: 1; (ii) nucleotides 142-948 of SEQ ID NO: 3; (iii) nucleotides 142-948 of SEQ ID NO: 5; and (iv) an amino acid sequence encoded by a nucleotide sequence at least 95% identical to any of (i)-(iii); (b) an amino acid sequence selected from among (v) amino acids 69-337 of SEQ ID NO: 2; (vi) amino acids 48-316 of SEQ ID NO: 4; (vii) amino acids 48-316 of SEQ ID NO: 6; and (viii) an amino acid sequence at least 95% identical to any of (iv)-(vi); or (c) an amino acid sequence that is immunologically cross-reactive with an antibody that specifically binds to a polypeptide consisting of an amino acid sequence as set forth in any one of (a) and (b), wherein the GRP94 ligand binding domain (LBD) polypeptide binds a GRP94 ligand selected from the group consisting of 4,4'-dianilino-1,1-binaphthyl-5,5-disulfonic acid (bis-ANS) and N-ethylcarboxamidoadenosine (NECA). Also disclosed are isolated polynucleotides encoding the disclosed polypeptides and methods of using the same to identify modulators of Hsp90 proteins.
|
