发明名称 |
Synthesis of oligonucleotides |
摘要 |
<p>A process for the synthesis in solution phase of a phosphorothioate triester is provided. The process comprises the solution phase coupling of an H-phosphonate with an alcohol in the presence of a coupling agent to form an H-phosphonate diester. The H-phosphonate diester is oxidised in situ with a sulfur transfer agent to produce the phosphorothioate triester. Preferably, the H-phosphonate and alcohol are protected nucleosides or oligonucleotides. Oligonucleotide H-phosphonates which can be used in the formation of phosphorothioate triesters are also provided.</p> |
申请公布号 |
EP1325927(A3) |
申请公布日期 |
2008.07.02 |
申请号 |
EP20030007651 |
申请日期 |
1998.08.10 |
申请人 |
AVECIA BIOTECHNOLOGY INC |
发明人 |
REESE, COLIN BERNHARD;SONG, QUANLAI |
分类号 |
C07H21/00;C12Q1/68;C07D207/48;C07D265/32;C07D265/33;C07F9/165;C07F9/18;C07F9/655;C07H1/00;C07H19/10;C07H19/20;C07H21/02;C07H21/04 |
主分类号 |
C07H21/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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