| 摘要 |
A full-length cDNA is obtained by analyzing the partial amino acid sequence of purified endo-beta-galactosidase C, constructing primers based thereon, effecting PCR with the use of the genomic DNA of Clostridium perfringens as a template to thereby obtain a fragment of cDNA encoding endo-beta-galactosidase, effecting cassette PCR by using the cDNA fragment thus obtained to thereby obtain the 5'-terminal and 3'-terminal domains, and further effecting PCR with the use of primers constructed on the basis of the data acquired above.
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