Isolated mutant serum albumin which has been mutated such that it displays an altered metal binding affinity and/or physiological other characteristic(s) with respect to a native albumin

摘要

An isolated mutant human serum albumin comprising the amino acid sequence: DAHKSEVAHRFKDLGEENFKALVLIAFAQX5LQQCPFEDHV KLVNEVTEFAKTCVADESAENCDKSLX1TLFGDKLCTVATL RETYGEMADCCAKQEPERX2X8CFX6QHKDDNPNLPRLVRPE VDVMCTAFHDNEETFLKKYLYEIARRX9PYFYAPELLFFAKR YKAAFTECCQAADKAACLLPKLDELRDEGKASSAKQRLKC ASLQKFGERAFKAWAVARLSQRFPKAEFAEVSKLVTDLTK VX10TECCX3X7X4LLECADDRADLAKYICENQDSISSKLKEC CEKPLLEKSX11CIAEVENDEMPADLPSLAADFVESKDVCKN YAEAKDVFLGMFLYEYARRHPDYSVVLLLRLAKTYETTLE KCCAAADPHECYAKVFDEFKPLVEEPQNLIKQNCELFEQLG EYKFQNALLVRYTKKVPQVSTPTLVEVSRNLGKVGSKCCK HPEAKRMPCAEDYLSVVLNQLCVLHEKTPVSDRVTKCCTES LVNRRPCFSALEVDETYVPKEFNAETFTFHADICTLSEKERQ IKKQTALVELVKHKPKATKEQLKAVMDDFAAFVEKCCKAD DKETCFAEEGKKLVAASQAALGL wherein X1, is other than H; X2 is other than N, X3 is other than H, X4 is other than D; X5 is other than Y; X6 is other than L; X7 is other than G, X8 is other than E, X9 is other than H, X10 is other than H, and/or X11 is other than H, such that said mutant displays an altered metal binding affinity with respect to native human serum albumin.