Antifungal compounds and agents able to inhibit transport of the GPI-anchored protein to the cell wall

摘要

Disclosed are antifungal compounds and agents, including compounds isolated using a screening method comprising: (a) contacting a test sample with a fungus that is overexpressing a protein; (b) detecting the amount of transport of a GPI-anchored protein to the cell wall in the fungus; and (c) selecting a compound that diminishes the amount of transport of the GPI-anchored protein to the cell wall detected in step (b) as compared to the amount of transport detected when the test sample was contacted with a fungus that is not overexpressing said protein, wherein said protein is a protein having an activity to confer resistance of a fungus against a disclosed compound when the DNA encoding the protein is over expressed in the fungus, wherein the DNA is selected from a disclosed group, or wherein said protein is a protein having an activity to decrease the amount of a GPI-anchored protein in the cell wall of a fungus due to a defect in the function of the DNA, wherein the encoding DNA is selected from a disclosed group.