| 摘要 |
<p>Disclosed is a method for selecting potyvirus-resistant plants, comprising the detection in the plants to be tested: - of the presence or of the absence of an eIF4E protein (hereinafter referred to as: "wild-type eIF4E protein") comprising a region defined by sequence (I) below DX1X2X3X4KSX5QX6AWGSSX7RX8X9YTFSX10VEX11FWX12X13YNNIHX14PSKLX15X16GAD in which: - X1, X2, X3, X4, X6, X7, X8, X9, X10, X11, X12, X13, X15 and X16 each represent a neutral amino acid; - X5 and X14 represent a basic amino acid; - X11 represents an acidic amino acid; - D, K, S, Q, A, W, G, R, Y, T, F, V, E, N, I, H, P and L have their usual 1-letter code meaning, or of a nucleotide sequence encoding said protein; - of the presence or of the absence of a mutant eIF4E protein comprising a region derived from that defined by sequence (I) by substitution of at least one neutral amino acid of said sequence (I) with a charged amino acid, and/or substitution of at least one charged amino acid of said sequence (I) with a neutral amino acid or an amino acid having an opposite charge, or of a sequence encoding said protein; - and the selection of the plants in which a mutant eIF4E protein or a nucleotide sequence encoding said protein is detected, and in which no wild-type eIF4E protein or sequence encoding said protein is detected.</p> |
