METHOD OF AMPLIFYING NUCLEIC ACID AND METHOD OF ANALYZING NUCLEIC ACID BY USING THE SAME

摘要

It is intended to prevent an increase in the amplification error occurring in the course of nucleic acid amplification to thereby give an amplification product at a high reproducibility. A nucleic acid amplification method characterized in that a nucleic acid to be amplified is amplified via two amplification steps, i.e., the first step of amplifying a single chain alone and the subsequent step of amplifying a chain being complementary to the amplification product. In the amplification, use is made of a first primer to be used in the amplification of the first step and a second primer to be used in the amplification of the second step. These primers may be used separately. Alternatively, they may be designed so as to have different stringencies and used simultaneously.