Method for determination of nucleated red blood cells and leukocytes in a whole blood sample in an automated hematology analyzer

摘要

A method for enumerating white blood cells and nucleated red blood cells. The method comprises the steps of: (b) providing a lysed sample of whole blood; (b) introducing the lysed sample to a light-scattering multi-angle depolarizing flow cytometer; (c) removing depolarizing interference, e.g., lipid droplets and other measured particles; (d) differentiating nucleated red blood cells and noise from white blood cells in the absence of depolarizing interference; (e) differentiating nucleated red blood cells from noise in the absence of depolarizing interference and white blood cells; and (f) differentiating possible platelet clumps.