发明名称 |
ORGANIC COMPOUNDS |
摘要 |
<p>The present invention relates to a new process for the production of a polypeptide of interest, or of a precursor thereof, wherein the protein or the precursor are expressed in a host cell comprising a cell wall and at least one cell membrane, followed by extraction from the intact host cell by partial permeabilization of the cell membrane under non-denaturing conditions. During extraction the polypeptide of interest, or the precursor thereof, which is expressed in at least partially correctly folded form, passes through the cell wall and the cell membrane.</p> |
申请公布号 |
WO2008074724(A1) |
申请公布日期 |
2008.06.26 |
申请号 |
WO2007EP63900 |
申请日期 |
2007.12.13 |
申请人 |
LEK PHARMACEUTICALS D.D.;MENART, VIKTOR;JESENKO, ANA;PRERADOV VASLE, TATJANA |
发明人 |
MENART, VIKTOR;JESENKO, ANA;PRERADOV VASLE, TATJANA |
分类号 |
C07K1/14;C07K1/36;C12N1/06;C12P21/00 |
主分类号 |
C07K1/14 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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