发明名称 |
SELECTIVE POSTTRANSLATIONAL MODIFICATION OF PHAGE-DISPLAYED POLYPEPTIDES |
摘要 |
The invention relates to posttranslational modification of phage-displayed polypeptides. These displayed polypeptides comprise at least one unnatural amino acid, e.g., an aryl-azide amino acid such as p-azido-L-phenylalanine, or an alkynyl-amino acid such as para-propargyloxyphenylalanine, which are incorporated into the phage-displayed fusion polypeptide at a selected position by using an in vivo orthogonal translation system comprising a suitable orthogonal aminoacyl-tRNA synthetase and a suitable orthogonal tRNA species. These unnatural amino acids advantageously provide targets for posttranslational modifications such as azide-alkyne [3+2] cycloaddition reactions and Staudinger modifications. |
申请公布号 |
EP1934343(A2) |
申请公布日期 |
2008.06.25 |
申请号 |
EP20060825758 |
申请日期 |
2006.10.11 |
申请人 |
THE SCRIPPS RESEARCH INSTITUTE |
发明人 |
TSAO, MENG-LIN;TIAN, FENG;SCHULTZ, PETER |
分类号 |
C12N15/10;C12N7/00;C12N9/00 |
主分类号 |
C12N15/10 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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