| 摘要 |
An HIV NC(nucleocapsid) protein expression vector is provided to express a wild type NC protein in an animal cell and improve the expression efficiency of the NC protein compared with conventional techniques. An HIV NC protein expression vector is characterized in that at least one sequence selected from the group consisting of an SV40 19s mRNA intron sequence, a mutated SV40 16S mRNA intron sequence and a beta-globin intron sequence, an HIV NC gene, and an mRNA stability element selected from the group consisting of RRE(Rev response element), WPRE(woodchuck posttranscriptional regulatory element), beta-actin 3'-untranslated regions and RSV(Rous Sarcoma Virus) stability element are linked in sequence. A method for producing the HIV NC protein comprises a step of culturing a cell transformed by the vector, wherein the cell is COS-7 or 293T cell.
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