| 摘要 |
A method of imaging fluorescence, in which an inverted fluorescence imaging apparatus provided with an immersion objective is used to image an observation phosphor for a constant term, includes an imaging position locating step (S1) of locating a vessel incorporating the observation phosphor at an imaging position of the fluorescence imaging apparatus; a liquid pouring step (S2) of pouring a preset amount of liquid between a top portion of the immersion objective in the fluorescence imaging apparatus and the vessel to bring about a charged state; an imaging step (S3) of imaging the observation phosphor in the vessel in a state where a space between the top portion of the immersion objective and the vessel is charged with the liquid; and a repeated control step (S4) of repeating a series of operations including the liquid pouring step (S2) of a single operation and the imaging step (S3) of a preset number of times of operations performed after the liquid pouring step (S2) of the single operation, at preset time intervals.
|
