METHOD OF PREPARATION OF LIQUID OR DRY CONCENTRATE OR BIFIDOBACTERIA FERMENT

摘要

FIELD: technological processes; medicine. ^ SUBSTANCE: method of preparation of liquid or dry concentrate or Bifidobacteria ferment includes introduction of inoculation dose of Bifidobacteria culture Bifidobacterium bifidum 8-3 (" ±- 1248"), Bifidobacterium longum "" (" ±-1252"), Bifidobacterium adolescentis Bl (" ±-1243") into nutrient medium. The medium contains, (mass %): lactopeptone 5.0-7.0; muriatic cysteine 0.001-0.005; autolysate or extract of bakery yeast 2.0-4.0; non-fat milk with content of non-fat milk solids of not more than 8.0% - the rest up to 100%. Biomass of bifidobacteria is grown at (37.0-38.0)±0.5°C until concentrate of bifidobacteria is obtained with at least 1011 CFU/ml. The product is cooled and packaged, or protective medium is introduced into it, and it is dried liophilically in sterile conditions. ^ EFFECT: provides for the possibility of preparation of stable uniform biomass of bifidobacteria for production of preparations with high bifidobacteria titre. ^ 5 cl, 5 tbl, 4 ex