发明名称 |
Protein fragment complementation assays in whole animals applications to drug efficacy, adme, cancer biology, immunology, infectious disease and gene therapy |
摘要 |
The instant invention describes a method for detecting protein-protein interactions in living organisms and/or cells, said method comprising: (a) synthesizing probe protein fragments from a protein which enables fluorescent or luminescent detection by dissecting the gene coding for the fluorescent or luminescent protein into a least two fragments; (2) constructing fusion proteins consisting of the probe protein fragments linked to protein domains that are to be tested for interactions; (3) coexpressing the fusion proteins; and (d) detecting reconstitution of the fluorescence or luminescence signal.
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申请公布号 |
US2008145315(A1) |
申请公布日期 |
2008.06.19 |
申请号 |
US20070987694 |
申请日期 |
2007.12.04 |
申请人 |
ODYSSEY PHARMACEUTICALS, INC. |
发明人 |
MICHNICK STEPHEN WILLIAM WATSON;MACDONALD MARNIE;WESTWICK JOHN K. |
分类号 |
A61K49/00;A61P43/00;C07K14/435;C12N15/10;G01N33/53;G01N33/542;G01N33/573;G01N33/58;G01N33/68 |
主分类号 |
A61K49/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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