发明名称 |
PURIFICATION PROCESS FOR COAT PROTEIN OF RNA BACTERIOPHAGES |
摘要 |
<p>The invention relates to aprocess for purifying coat protein of an RNA bacteriophage, wherein said process comprises the steps of disassembling a virus-like particle of an RNA bacteriophage and subsequently purifying said coat protein. The process described herein is designed to be scaled up to produce up to 100 g coat protein and more per batch, and it allows the efficientremoval of RNA and host cell derived impurities, typically below the quantification level. The invention further relates to purified coat protein of RNA bacteriophages, wherein said purified coat protein typically comprises less than 0.5 µg RNA, less than 0.4 IU of endotoxin, less than 5 ng host cell protein, and less than 1.5 ng of host cell DNA per 100 µg of said coat protein.</p> |
申请公布号 |
WO2008071774(A1) |
申请公布日期 |
2008.06.19 |
申请号 |
WO2007EP63907 |
申请日期 |
2007.12.13 |
申请人 |
CYTOS BIOTECHNOLOGY AG;GUENTHER, CONSTANZE;PROBA, KARL;RICHTER, SUSANNE;TOPELL, SIMON |
发明人 |
GUENTHER, CONSTANZE;PROBA, KARL;RICHTER, SUSANNE;TOPELL, SIMON |
分类号 |
C07K14/08;C12N7/02;C12N7/04 |
主分类号 |
C07K14/08 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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