| 摘要 |
<p>Disclosed is a method which can detect an abnormal prion more rapidly compared to a conventional method. In the method, a sample to be examined on whether or not the sample contains an abnormal prion is mixed with a fluorescently labeled normal prion to prepare a sample solution, and the sample solution is exposed to a reaction condition which enables an abnormal prion to convert a normal prion into an abnormal one. Subsequently, the fluorescence intensity of the fluorescent label attached to the fluorescently labeled normal prion in the sample solution is measured. Based on the measured value of fluorescence intensity, it is determined whether the fluorescently labeled normal prion is present in the sample solution in an unmodified form or in the form incorporated in an aggregate. When it is determined that the fluorescently labeled normal prion is incorporated in an aggregate, then it is concluded that the sample contains an abnormal prion.</p> |
