发明名称 |
BUFFER SOLUTION FOR ELECTROPORATION AND A METHOD COMPRISING THE USE OF THE SAME |
摘要 |
A buffer solution for suspending animal or human cells and for dissolving biologically active molecules in order to introduce the biologically active molecules into the cells using electric current. The buffer solution includes HEPES and at least 10 mmol*1<SUP>-1 </SUP>magnesium ions (Mg<SUP>2+</SUP>). The buffer solution has a buffer capacity of at least 20 mmol*1<SUP>-1</SUP>*pH<SUP>-1 </SUP>at a change in the pH from pH 7 to pH 8 and at a temperature of 25° C. The buffer solution also has an ionic strength of at least 200 mmol*1<SUP>-1</SUP>.
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申请公布号 |
US2008145938(A1) |
申请公布日期 |
2008.06.19 |
申请号 |
US20070931886 |
申请日期 |
2007.10.31 |
申请人 |
AMAXA AG |
发明人 |
RIEMEN GUDULA;LORBACH ELKE;HELFRICH JULIANA;SIEBENKOTTEN GREGOR;MULLER-HARTMANN HERBERT;ROTHMANN-COSIC KIRSTEN;THIEL CORINNA;WEIGEL MEIKE;WESSENDORF HEIKE;BROSTERBUS HELMUT;NIX MICHAEL |
分类号 |
C12N15/09;A61K35/12;A61K38/00;A61K47/00;A61K48/00;C12N13/00;C12N15/87;G01N33/50 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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