METHOD OF EVALUATING SKIN BARRIER FUNCTION, METHOD OF SCREENING MATERIAL FOR ENHANCING SKIN BARRIER FUNCTION USING THE EVALUATING METHOD, THE MATERIAL FOR ENHANCING SKIN BARRIER FUNCTION, AND COSMETIC CONTAINING THE MATERIAL FOR ENHANCING SKIN BARRIE

摘要

An object of the present invention is to provide a technology for developing an excellent material for enhancing skin barrier function and to provide a cosmetic which is developed based on the technology and has excellent ability to enhance the skin barrier function. The present invention relates to a method of evaluating skin barrier function, comprising: 1) culturing normal human epidermal cells in wells with a semipermeable membrane bottom; 2) adding a medium not containing metal ions to be measured to the above of cells in each well and a medium containing the metal ions to be measured to the below of the cells in each well, provided that the below is a side where the cells are adhered to the semipermeable membrane, and the above is an opposite side thereto, and culturing the cells under metal ion loaded condition; 3) measuring a concentration of the metal ions in each medium; and 4) calculating permeability of the metal ions in an epidermal cell layer based on the measured concentrations of the media to evaluate the skin barrier function.