ANTIBODY DETECTION METHOD INVOLVING OLIGONUCLEOTIDE ENHANCED COLLOIDAL GOLD SIGNAL

摘要

The present invention refers to a rapid immunochromatographic test device for antibody detection, comprising at least a first and a second conjugate releasing pad, wherein the first conjugate pad comprises a gold conjugated protein linked first oligonucleotide and a gold conjugated anti-antibody, and the second conjugate pad comprises a gold conjugated protein linked second oligonucleotide, which second oligonucleotide is complementary to the first oligonucleotide. The present invention further refers to a use of such test device for antibody detection in urine or saliva, e.g. HIV antibody. Embodiments of the test device are a test strip and a detection cup. The present invention also refers to a method for manufacturing such test device.