| 摘要 |
A device ( 1 ) for the carrying out of investigations on cell cultures ( 2 ) which take place in a liquid culture medium ( 4 ). The device possesses at least a receptacle ( 3 ) to contain the culture medium ( 4 ) including the cell culture ( 2 ), wherein one or more sensors ( 6 ) for measurement of the cell culture activities is provided and wherein the receptacle ( 3 ) has at least an opening for the adding and the removal of liquid culture medium ( 4 ) and the like. A separating element ( 7 ) can be introduced into the open upper part of the receptacle ( 3 ) and can be brought within the said receptacle ( 3 ) in a position close to the bottom ( 5 ), in which the said separating element ( 7 ) makes a boundary forming a partial space ( 8 ) which has a small volume in relation to the entire volume of the receptacle ( 3 ). Further, a flow channel ( 9 ) is provided, which first, communicates with the small volume partial space ( 8 ), and second, communicates with the said reservoir ( 14 ). This flow channel ( 9 ) and/or the sensors ( 6 ) are placed in the area of the small volume partial space ( 8 ). By means of the capability for adjustment of the separating element ( 7 ) as to its distance from the bottom of the receptacle ( 3 ), in the neighborhood of the cell culture ( 2 ) a microreaction volume exactly fitting the current requirements can be adjusted. By means of the flow channel ( 9 ), the cell culture ( 2 ) in the small volume partial space ( 8 ) stands in fluid communication with that in the reservoir. For the regeneration of the cell culture solution in the small volume partial space, the separating element ( 7 ) can be elevated and depressed, or it is possible, to inject into the small volume partial space of the receptacle ( 3 ) a quantity of liquid and by means of a through-flow channel. In this case, a convective mixing of fresh and spent medium takes place through the flow channel ( 9 ) between the small volume partial space and the reservoir ( 14 ).
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