METHOD FOR THE ENANTIOSELECTIVE ENZYMATIC REDUCTION OF SECODIONE DERIVATIVES

摘要

The invention relates to a method for the enantioselective enzymatic reduction of secodione derivatives of general formula (I), wherein the ring structures comprise no, one, or several heteroatom/s, R₁ represents hydrogen or a C₁-C₄alkyl group, R₂ represents hydrogen, a C₁-C₈ alkyl group, or a protective OH- group known in prior art, such as an ester, R₃ represents hydrogen, a methyl group, or a halide, the structural element represents a benzene ring or a C₆ ring containing 0, 1, or 2 C-C double bonds, positions 6/7 or 7/8 optionally contain a double bond, and the carbon is independently substituted with hydrogen, a C₁-C₄ alkyl group, a halide, or a phenyl group in positions 1, 2, 4, 5, 6, 7, 8, 9, 11, 12, and 16. In said method, the secodione derivative is reduced by means of an oxidoreductase/dehydrogenase in the presence of NADH or NADPH as a cofactor. According to the invention, the secodione derivative is used at a concentration of =10 g/l in the reaction batch, and the oxidized NAD or NADP cofactor formed by the oxidoreductase/dehydrogenase is continuously regenerated.