NOVEL USE OF OIP5 GENE

摘要

An OIP gene is provided to diagnose cancer by detecting overexpression of gene or protein in cells by using the expression increase properties in cancer cells, screen anticancer agent by measuring increase or decrease of gene expression by a candidate anticancer material and treat cancer by using OIP gene inhibitors. A composition for screening anticancer agent comprises an OIP gene or a protein expressed from the OIP gene. A screening method of anticancer agent comprises the steps of: contacting the anticancer agent-screening composition as a target material with a candidate anticancer material; analyzing the reaction between the target material and the candidate anticancer material; and deciding the activity of candidate anticancer material to increase or inhibit the gene expression. The siRNA(small interfering RNA) of OIP gene comprises the nucleotide sequence corresponding to mRNA derived from 19-30 consecutive nucleotide sequences in at least on sequence selected from SEQ ID NOs:19, 20, 21, 22, 23, 24, 25 and 26, and has the nucleotide sequence of SEQ ID NO:3 or 4. The shRNA(short hairpin RNA) of OIP gene comprises the nucleotide sequence corresponding to mRNA derived from 19-30 consecutive nucleotide sequences in at least on sequence selected from SEQ ID NOs:19, 20, 21, 22, 23, 24, 25 and 26, and has RNA sequence of SEQ ID NO:5 and reversely complementary RNA sequence connected thereto with 3-10 base of loop area. A composition for treating cancer contains siRNA of OIP gene or shRNA of OIP gene as OIP gene inhibitor.