发明名称 |
METHOD FOR PRODUCING CDNA AND RNA CHAINS AND NUCLEOTIDE-IMMOBILIZED SUPPORT |
摘要 |
A method of synthesizing a cDNA chain using an insoluble carrier having on the surface thereof a polymer substance containing a first unit having a group derived from a phosphoric ester composing the hydrophilic portion of phospholipid, and a second unit having a group derived from carboxylic acid having an electron-attractive substituent bound to a carbonyl group, which includes immobilizing a polynucleotide for DNA elongation; bringing a solution containing an RNA fragment, nucleotide monomers, and a reverse transcriptase or an enzyme having polymerase activity into contact with the surface of the insoluble carrier; and allowing the polynucleotide for DNA elongation immobilized on the surface of the carrier to elongate using the RNA fragment contained in the solution as a template, to thereby form a single-strand cDNA.
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申请公布号 |
EP1930422(A1) |
申请公布日期 |
2008.06.11 |
申请号 |
EP20060782550 |
申请日期 |
2006.08.09 |
申请人 |
SUMITOMO BAKELITE COMPANY, LTD.;KINOSHITA, KENJI |
发明人 |
KINOSHITA, KENJI;YAKABE, TORU;FUJIMOTO, KENTARO;YOKOYAMA, KANEHISA;FUJIWARA, KAZUHIKO |
分类号 |
C12N15/10;C12Q1/68 |
主分类号 |
C12N15/10 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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