发明名称 |
Interface patch clamping |
摘要 |
The invention provides a novel development of the conventional patch clamp technique for measurement of whole cell electrical activity. The invention provides for one or more cell or cells to be suspended in a liquid medium at a liquid/air interface (by virtue of the effect of surface tension at the interface) whereby the cell or cells are accessible at the interface to a microstructure electrode (such as a pipette tip) to which a cell can attach to form an electrical seal, for the purpose of whole cell voltage clamp recording. According to the invention the electrode can be caused to form a high resistance electrical seal with a cell suspended in the liquid at the liquid/air interface without the need to press the cell against a solid support surface. The invention also provides apparatus for carrying out the interface patch clamp technique and control logic for operating a computer to carry out the interface patch clamp technique.
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申请公布号 |
US7384733(B1) |
申请公布日期 |
2008.06.10 |
申请号 |
US19990857456 |
申请日期 |
1999.12.06 |
申请人 |
XENTION DISCOVERY LIMITED |
发明人 |
BYRNE NICHOLAS GERARD;OWEN DAVID GERAINT |
分类号 |
C12Q1/00;G01N33/483;C12M1/34;C12M3/00;C12Q1/02;G01N1/10;G01N27/28;G01N27/30;G01N27/416;G01N33/487 |
主分类号 |
C12Q1/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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