METHOD FOR CONTROLLING THE GROWTH OF CELL CULTURE

摘要

The present invention provides a method for controlling the growth of mic robes grown in a medium to high cell-densities by the fed-batch technology, wherein a two-phase system, having a liquid phase (cultivation medium) and a solid or gel phase, the solid or gel phase provides a source of substrate-d elivering polymer which is turned by an enzyme in a controlled way into a gr owth-limiting substrate or pH adjusting agent. The present invention also pr ovides a method for restricting the synthesis of growth-limiting metabolites and preventing oxygen-depletion in microbial cell cultures.