Oil-immersion, soft-print array replication

摘要

A method of replicating a DNA microarray in which a multi-drop transfer plate is loaded with PCR master-mix and then moved into close proximity with a master array so that a portion of the PCR master mix is transferred to the master array. The master array is immersed in oil so that complementary DNA may be produced by one or more steps of a PCR reaction. A replica substrate loaded with water drops is then brought into close proximity with the master array so that a portion of complementary DNA is transferred onto the replica substrate. After cleaning any residual oil from replica substrate, it is ready for use.