| 摘要 |
A method for adding a first and a second functional nucleic acid sequence to a reaction mixture, in particular an amplification reaction mixture in a predetermined stoichiometry and/or at a predetermined point in time, said method comprising adding to the reaction mixture an oligonucleotide comprising a first and a second functional nucleic acid sequence separated by a spacer sequence, said spacer sequence comprising a region which, when double stranded, comprises a cleavable region, forming a cleavable double stranded region within the spacer region of said oligonucleotide, and cleaving the double stranded region within said oligonucleotide. Oligonucleotides for use in the method, and comprising a first and a second functional nucleic acid sequence, such as primers or probes used in an amplification reaction, separated by a spacer sequence, is also provided.
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