发明名称 |
Compositions comprising mixtures of positional PEG isomers of PEGylated G-CSF |
摘要 |
A method for increasing the stability and uniformity of a PEGylated G-CSF polypeptide having at least one PEG moiety attached to the epsilon amino group of a lysine residue or the N-terminal amino group and at least one PEG moiety attached to a hydroxyl group, comprising subjecting the polypeptide to an elevated pH of above 8.0 for a period of time suitable to remove PEG moieties attached to a hydroxyl group, and reducing the pH to about 8.0 or lower; as well as PEGylated G-CSF polypeptides and compositions produced according to the method and methods for increasing neutrophil levels in a patient using the PEGylated G-CSF polypeptides and compositions.
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申请公布号 |
US7381805(B2) |
申请公布日期 |
2008.06.03 |
申请号 |
US20060420546 |
申请日期 |
2006.05.26 |
申请人 |
MAXYGEN HOLDINGS, LTD. |
发明人 |
GERMANSEN CARSTEN;SONI BOBBY;RASMUSSEN GRETHE |
分类号 |
C07K14/53;A61K38/24;A61K47/36 |
主分类号 |
C07K14/53 |
代理机构 |
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地址 |
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