发明名称 |
METHOD TO PRODUCE STERILE MALE FLOWERS AND PARTENOCARPIC FRUITS BY GENETIC SILENCING, ASSOCIATED SEQUENCES AND VECTORS CONTAINING SAID SEQUENCES |
摘要 |
Genes VvPI from Vitis vinifera cv. Cabernet Sauvignon and LePI from Lycopersicon esculentum are described; together with the use of these genes to produce sterile male flowers and seedless or parthenocarpic fruits. Silencing vectors that comprise these sequences or a part thereof are disclosed. The methods of the invention are directed to producing sterile male flowers and parthenocarpic fruits by genetic silencing, and includes: obtaining the codifying sequence of Pistillata (PI)-homologous genes from the target species; analyzing the expression of the sequence obtained in step (a) to test its expression according to the pattern described for Pistillata genes; analyzing the complementation of PI-gene mutant with the PI sequence obtained from the target species, to assess that the obtained sequence fulfills the function of a PI gene; making a genetic silencing construct that comprises a region of the codifying sequence of PI in a plant expression vector; incorporation of the constructed vector into Agrobacterium tumefaciens; transforming target plants with Agrobacterium tumefaciens modified with the silencing vector and selecting said transformed plants; and checking the absence of Agrobacterium contamination and corroborating transgenic plants by transgene amplification. |
申请公布号 |
CA2615249(A1) |
申请公布日期 |
2008.06.01 |
申请号 |
CA20072615249 |
申请日期 |
2007.11.29 |
申请人 |
PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE |
发明人 |
ARCE JOHNSON, JORGE PATRICIO;FEDERICI NOE, FERNAN;MEDINA AREVALO, MARIA CONSUELO;CADAVID, LABRADA, AGNES;POUPIN SWINBURN, MARIA JOSEFINA |
分类号 |
C12N15/29;A01H1/00;A01H3/00;A01H5/00;C07K14/415;C12N15/82 |
主分类号 |
C12N15/29 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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