OLIGONUCLEOTIDE AND DNA CHIP FOR DETECTING RESPIRATORY VIRAL PATHOGEN

摘要

A DNA chip is provided to detect 8 kinds of respiratory virus at the same time, show excellent sensitivity and specificity and diagnose infection by PIV1, PIV2, PIV3, RSV A, RSV B, HcoV(229E), HcoV(OC43) and SARS virus rapidly by omitting a subsequent PCR process, thereby being very usefully used for preparing a diagnosis reagent or a diagnosis kit for the virus infection. An oligonucleotide DNA chip for detecting respiratory virus is characterized in that oligonucleotide probes having nucleotide sequences of SEQ ID : NOs. 1 to 8 are immobilized on a slide, wherein each of the probes is specifically coupled to a HA gene of PIV1, a HA gene of PIV2, a HA gene of PIV3, an N gene of RSV A, an N gene of RSV B, an M gene of HcoV(229E), an M gene of HcoV(OC43), and an N gene of SARS. A kit for detecting the respiratory diseases comprises: the oligonucleotide DNA chip; and a marking means which detects hybridization reaction of cDNA synthesized from a sample RNA obtained from the probe of the DNA chip and a test body, wherein the marking means a fluorescent material including cy5-dUTP.