| 摘要 |
PROBLEM TO BE SOLVED: To solve the problem wherein the dependence of the degree of change in the light quantity on the film thickness of a light-transmitting part of a sample support, its refractive index, incident angle of incident light, wavelengths, and light quantity, when a trace amount of small optical changes on the sample support are to be captured as the change in the light quantity has not been disclosed at all in the conventional reaction analysis apparatuses that utilize polarization. SOLUTION: In a reaction analysis method, the light reflected from a first optical element for making polarized light fall incident on a part of a biological sample, and from a second optical element for reflecting light by rotating the plane of polarization of p-polarized light and s-polarized light of reflected light reflected at the part of the biological sample by nπ+π/2 (n: is an integer of 0, 1, ...) is made incident on a second sample and is quenched by an analyzer, and the biological sample is analyzed. The sample support has a light non-transmitting layer; a light-transmitting layer; and a reaction layer on the biological sample, and the layer thickness of the reaction layer on the biological sample changes, prior to and after reaction. Reflectivity of the s-polarized light at an angle of incidence to the sample support is 0.25 or smaller. By detecting the changes in the light quantity, by reversing of the positive and negative phase differences of the s-polarized light and the p-polarized light in the light-transmitting layer, the reaction results are acquired. COPYRIGHT: (C)2008,JPO&INPIT
|
