MODIFIED GP140 ENVELOPE POLYPEPTIDES OF HIV-1 ISOLATES, COMPOSITIONS, STABILIZED TRIMERIC COMPLEXES, AND USES THEREOF

摘要

This invention provides a modified gpl40 envelope polypeptide of an HIV-1 isolate comprising a gpl20 polypeptide portion comprising consecutive amino acids and a gp41 ectodomain polypeptide portion comprising consecutive amin o acids, said gp41 ectodomain polypeptide portion being modified to comprise isoleucine (I) at an amino acid position equivalent to amino acid position 535; glutamine (Q) at an amino acid position equivalent to amino acid positi on 543; serine (S) at an amino acid position equivalent to amino acid positi on 553; lysine (K) at an amino acid position equivalent to amino acid positi on 567; and arginine (R) at an amino acid position equivalent to amino acid position 588, the amino acid positions being numbered by reference to the HI V-1 isolate KNH1144. This invention also provides nucleic acids encoding suc h a polypeptide, vectors, host cells, trimeric complexes and compositions th ereof. Also provided are antibodies generated against the modified polypepti des and trimeric complexes, and methods of using the modified polypeptides, compositions and trimeric complexes.