Bead based receptor biology

摘要

A method for capturing activated receptor signaling complexes from live cells, utilizing bead based biology wherein live cells are contacted with ligand coated beads to form bead binding sites and thereby initiating formation of a ligand-receptor complex at said bead binding site; and a process for distinguishing and confirming non-specifically bound proteins from specifically bound receptor complexes by utilization of one or more methods of biochemical or biophysical analysis, thereby providing, in a preferred embodiment, a utilization of confocal microscopy and proteomic mass spectroscopy.