| 摘要 |
<P>PROBLEM TO BE SOLVED: To provide methods for producing a polypeptide as inclusion bodies in bacterial host cells. <P>SOLUTION: The methods are carried out by forming a gene construct comprising the genetic sequence encoding a polypeptide operatively linked to that of an inclusion partner protein, such as E. coli thioredoxin or a modified E. coli thioredoxin, such that host cells comprising the gene construct produce the polypeptide as inclusion bodies. The method of the invention facilitates the rapid isolation and purification of recombinant proteins. In addition, the present method may be useful for producing polypeptides or proteins which are small and are typically difficult to express as well as those proteins that are toxic to host cells such as E. coli. The methods produce protein molecular weight ladders for use in protein gel electrophoresis. <P>COPYRIGHT: (C)2008,JPO&INPIT |
