IMPROVED HYBRIDISATION OF NUCLEIC ACIDS

摘要

A method of reducing nucleotide mis-pairing in hybridisation between a probe oligonucleotide and a target DNA, the method comprising providing at least one oligonucleotide probe of about 7 to about 25 nucleotides, providing a sample comprising target DNA, wherein the DNA is prepared so as to comprise fragments of up to about 100 bases, incubating the DNA with the at least one oligonucleotide probe under conditions suitable to enable hybridisation between probe and target DNA, removing unbound DNA, and detecting DNA hybridised to the at least one oligonucleotide probe, wherein either or both of the DNA and the at least one oligonucleotide probe comprises one or more modified nucleotides, and wherein the DNA of the sample is optionally labelled with a detectable moiety.