发明名称 |
Modified xylanases exhibiting improved expression |
摘要 |
A modified Family 11 xylanase enzyme comprising a sequence that introduces a functional consensus glycosylation site is provided. Non-limiting examples of introduced glycosylation sites include mutation of the amino acid at position 34, 131, 180, 182, or a combination thereof, to an asparagine. The indicated amino acid position in the Family 11 xylanase is determined from sequence alignment of the xylanase of interest with that of a Trichoderma reesei xylanase II amino acid sequence. The introduced consensus glycosylation site facilitates increased expression efficiency of the modified xylanase when compared to the expression efficiency of a corresponding xylanase from which the modified xylanase was derived, using similar host strains and growth conditions. |
申请公布号 |
ZA200608088(B) |
申请公布日期 |
2008.05.28 |
申请号 |
ZA20060008088 |
申请日期 |
2005.03.24 |
申请人 |
IOGEN BIO-PRODUCTS CORPORATION |
发明人 |
WHITE, THERESA;GIROUX, GENEVIEVE R;WALLACE, KATIE E A |
分类号 |
A21D8/04;A23K;A23K1/165;A23K1/18;A23K3/00;A23L;A23L1/03;C12N;C12N1/15;C12N1/18;C12N9/24;C12N15/56;C12N15/74;D21H;D21H17/22 |
主分类号 |
A21D8/04 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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