| 摘要 |
The present invention is related to a method for determining the effect of a test agent on a target molecule comprising the following steps:
a) providing the target molecule,
b) providing a target molecule interacting entity,
c) providing the test agent,
d) combining the target molecule, the test agent and the target molecule interacting entity,
whereupon the test agent induces a change in the conformational state of the target molecule and
said change in the conformational state of the target molecule results in a change in affinity of the target molecule for the target molecule interacting entity,
e) determining the amount of the target molecule interacting entity which interacts with the target molecule, or, alternatively, determining the amount of the target molecule which interacts with the interacting entity, and optionally
f) determining the effect of the test agent on the target protein,
whereby the target molecule is a protein from the family of nuclear receptors and either the target molecule or the target molecule interacting entity is immobilized, preferably immobilized to a surface .
|
