发明名称 |
METHODS AND COMPOSITIONS FOR SECRETION OF HETEROLOGOUS POLYPEPTIDES |
摘要 |
The instant invention discloses the unexpected result that mutant signal sequences with reduced translational strength provided essentially complete processing and high levels of expression of a polypeptide of interest as compared to wild type signal sequences, and that many mammalian polypeptides require a narrow range of translation levels to achieve maximum secretion. A set of signal sequence vectors provides a range of translational strengths for optimizing expression of a polypeptide of interest.
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申请公布号 |
CA2213813(C) |
申请公布日期 |
2008.05.27 |
申请号 |
CA19962213813 |
申请日期 |
1996.02.27 |
申请人 |
GENENTECH, INC. |
发明人 |
YANSURA, DANIEL G.;SIMMONS, LAURA C. |
分类号 |
C12N15/09;C12N15/67;C07K14/245;C12N1/21;C12N15/31;C12N15/62;C12N15/70;C12P21/00;C12P21/02;C12R1/19 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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