РЕКОМБИНАНТНАЯ ПЛАЗМИДНАЯ ДНК PAS-2, КОДИРУЮЩАЯ ПОЛИПЕПТИД ПРОИНСУЛИНА ASPART ЧЕЛОВЕКА, И ШТАММ БАКТЕРИЙ ESCHERICHIA COLI BAS2 - ПРОДУЦЕНТ РЕКОМБИНАНТНОГО ПРОИНСУЛИНА ASPART

摘要

FIELD: chemistry, pharmacology. ^ SUBSTANCE: plasmid pAS-2 is formed by HindIII/BamHI-fragment of plasmid pPINS07 incorporating a part of artificial gene encoding lgG-binding protein domain A from S. aureus and peptide His6GlySerArg, and HindIII/BamHI-fragment encoding proinsulin Aspart. Plasmid pAS-2 ensures in cells E. coli the biosynthesis of hybrid protein wherein B domain lineage of staphylococcal protein A Staphylococcus aureus is linked via peptide linker His6GlySerArg with amino acid sequence of human being proinsulin Aspart. The human being strain of bacteria Escherichia coli BAS2 produced by transforming the cells of the strain by plasmid pAS-2 produces the said hybrid protein. ^ EFFECT: production of human being insulin Aspart by simplified process with high yield. ^ 2 cl, 4 dwg, 4 ex