发明名称 |
METHOD OF TROPHOBLASTIC BETA-1-GLYCOPROTEIN RELEASE AND PURIFICATION |
摘要 |
FIELD: medicine; chemistry-pharmaceutical industry. ^ SUBSTANCE: trophoblastic beta-1-glycoprotein is released from serosity produced as a result of differential centrifugation of retroplacental human blood. Supernatant after first centrifugation is saturated with sodium chloride (0.1-1.0 mole/l) to prevent non-specific sorption and centrifuged once more with triton X-100 (0.01-0.05%) to virus inactivation. Then it is applied to affine sorbent that is sepharose with solid-phase monoclonal antibodies to trophoblastic beta-1-glycoprotein. Protein is eluated with 0.1 M glycine-HCI buffer solution, and after neutralization and in-depth analysis second affine chromatography is carried out on sepharose with modified protein G, released from streptococcus cell wall to prevent immunoglobulin contamination. Solution passed through column and trophoblastic beta-1-glycoprotein is been concentrating and dialyzing from saline and lyophilized. ^ EFFECT: increased output and purity of end product. ^ 2 ex |
申请公布号 |
RU2325171(C1) |
申请公布日期 |
2008.05.27 |
申请号 |
RU20070100406 |
申请日期 |
2007.01.09 |
申请人 |
RAEV MIKHAIL BORISOVICH;INSTITUT EHKOLOGII I GENETIKI MIKROORGANIZMOV URAL'SKOGO OTDELENIJA RAN |
发明人 |
RAEV MIKHAIL BORISOVICH;SHMAGEL' KONSTANTIN VLADIMIROVICH;RAEV ALEKSANDR BORISOVICH;CHERESHNEV VALERIJ ALEKSANDROVICH;DEMAKOV VITALIJ ALEKSEEVICH;BAKHMET'EV BORIS ARKAD'EVICH |
分类号 |
A61K35/16;A61K38/17 |
主分类号 |
A61K35/16 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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